5HBA

Globular Domain of Zebrafish Complement 1qA protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	50.347, 85.059, 95.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.855 - 2.050
R-factor	0.1838
Rwork	0.182
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pk6
RMSD bond length	0.007
RMSD bond angle	1.107
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.074	0.196
Number of reflections	26479
<I/σ(I)>	22.222	11.75
Completeness [%]	99.6	100
Redundancy	6.8	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	ammonium sulfate, PEG 3350