5HB7

Crystal structure of Chaetomium thermophilum Nup53 RRM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.7293
Spacegroup name	P 21 21 21
Unit cell lengths	34.990, 50.370, 60.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.586 - 0.819
R-factor	0.1186
Rwork	0.118
R-free	0.13150
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	0.800
Number of reflections	92712
<I/σ(I)>	29.3
Completeness [%]	88.4
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M HEPES (pH 7.0), 24 % (w/v) PEG 3350, 0.2 M potassium iodide