5HB6

Crystal structure of Chaetomium thermophilum Nup145N APD T994A mutant fused to Nup145C N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	43.018, 44.120, 45.336
Unit cell angles	99.76, 110.98, 105.91

Refinement procedure

Resolution	25.926 - 1.300
R-factor	0.1512
Rwork	0.151
R-free	0.17390
RMSD bond length	0.007
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.300
Number of reflections	66722
<I/σ(I)>	17.2
Completeness [%]	94.7
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M TRIS (pH 8.8), 32 % (w/v) PEG 4000, 0.2 M lithium sulfate