5HB4
Crystal structure of Chaetomium thermophilum Nup192
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.740, 53.500, 171.790 |
| Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
| Resolution | 47.739 - 3.200 |
| R-factor | 0.2345 |
| Rwork | 0.233 |
| R-free | 0.26470 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.208 |
| Number of reflections | 27800 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 98.6 |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 294 | 0.1 M TRIS (pH 7.9), 6 % (w/v) PEG 4000, 5 % (v/v) polypropylene glycol |
