5HB1

Crystal structure of Chaetomium thermophilum Nup170 SOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0330
Spacegroup name	P 21 21 2
Unit cell lengths	96.070, 138.620, 89.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 4.007
R-factor	0.3087
Rwork	0.303
R-free	0.35240
RMSD bond length	0.003
RMSD bond angle	0.462
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	4.000
Number of reflections	10614
<I/σ(I)>	18.5
Completeness [%]	99.7
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	294	0.1 M HEPES (pH 7.1), 0.5 M ammonium sulfate