5HAU

Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	208.782, 449.030, 619.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.478 - 3.000
R-factor	0.2111
Rwork	0.208
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	49.658	3.180
High resolution limit [Å]	3.000	8.860	3.000
Rmerge	0.263	0.030	1.198
Rmeas	0.296	0.034	1.356
Total number of observations	5295862
Number of reflections	1140965	44887	181074
<I/σ(I)>	7.04	36.99	1.34
Completeness [%]	99.1	97.3	98.1
Redundancy	4.642
CC(1/2)		0.999	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME