5HAG
Structure of Chlamydia trachomatis effector protein ChlaDUB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 133.292, 133.292, 133.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.620 - 2.100 |
| R-factor | 0.1838 |
| Rwork | 0.183 |
| R-free | 0.20810 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.817 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.620 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.521 |
| Number of reflections | 23093 | |
| <I/σ(I)> | 11.1 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES (pH 7.5), 20% PEG 8000 |
