5HAF
Structure of Salmonella enterica effector protein SseL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 113.009, 113.009, 166.602 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.386 - 2.700 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22810 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.669 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.390 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.122 | 0.627 |
| Number of reflections | 33454 | |
| <I/σ(I)> | 7.9 | 2.4 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris, 1.6 M Dipotassium phosphate (pH 8.7) |
