5HAE

Crystal structure of LTBP1 LTC4 complex collected on an in-house source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-10-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	34.269, 58.388, 36.018
Unit cell angles	90.00, 103.75, 90.00

Refinement procedure

Resolution	34.986 - 2.002
R-factor	0.1493
Rwork	0.146
R-free	0.20240
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.064	0.045	0.136
Rmeas	0.074	0.052	0.157
Rpim	0.036	0.026	0.077
Total number of observations	35561
Number of reflections	9042
<I/σ(I)>	20.6
Completeness [%]	96.4	98.6	93.8
Redundancy	3.9	3.9	3.8
CC(1/2)		0.995	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M Tris, pH 8, 30% PEG 6000