5HAD

Crystal structure of intermembrane space region of chloroplast protein ARC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	60.479, 60.479, 91.678
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.718 - 2.238
R-factor	0.2032
Rwork	0.201
R-free	0.24310
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.654
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.238	2.240
Number of reflections	9790
<I/σ(I)>	68.8	13.9
Completeness [%]	100.0	99.8
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	ethylene glycol