5HAA

Crystal structure of mpy-RNase J, an archaeal RNase J from Methanolobus psychrophilus R15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 62 2 2
Unit cell lengths	162.508, 162.508, 180.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.033 - 2.904
R-factor	0.2399
Rwork	0.237
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zq4
RMSD bond length	0.006
RMSD bond angle	1.305
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1980)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.098	1.000
Number of reflections	31664
<I/σ(I)>	38.3	2
Completeness [%]	100.0	100
Redundancy	39	41.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	285	The crystal was grown in the solution containing 2M (NH4)2SO4 and 2%-5% glycerol.