5H9Y
Crystal structure of GH family 64 laminaripentaose-producing beta-1,3-glucanase from Paenibacillus barengoltzii complexed with laminarihexaose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-05-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0093 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.561, 65.191, 61.426 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 28.270 - 1.969 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h9x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.270 | 2.040 |
| High resolution limit [Å] | 1.969 | 1.970 |
| Rmerge | 0.090 | |
| Number of reflections | 33814 | |
| <I/σ(I)> | 11.21 | 3.41 |
| Completeness [%] | 93.5 | 64.54 |
| Redundancy | 6.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Se-derivatized PbBgl64A was prepared and concentrated to 15 mg ml-1 in buffer D contain 5 mM DL-Dithiothreitol (DTT), and optimized crystals were grown in drops containing 2 microliter of protein solution and 0.5 microliter of reservoir solution (14mM sodium cholate, 1.1M di-Ammonium Tartrate pH 7.0) at 293 K |
