5H9X

Crystal structure of GH family 64 laminaripentaose-producing beta-1,3-glucanase from Paenibacillus barengoltzii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	134.108, 65.810, 61.628
Unit cell angles	90.00, 105.44, 90.00

Refinement procedure

Resolution	32.317 - 1.910
R-factor	0.1948
Rwork	0.194
R-free	0.21660
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.199
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.320	1.970
High resolution limit [Å]	1.910	1.910
Rmerge	0.095
Number of reflections	36956
<I/σ(I)>	13.72
Completeness [%]	91.2	89.91
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Optimized crystals suitable for diffraction were grown in drops containing 2 microliter of protein solution and 0.5 microliter of reservoir solution (1.2M di-Ammonium Tartrate pH 7.0) at 293 K. The crystals were observed 10 days later.