5H9W
Crystal structure of Regnase PIN domain, form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.333, 113.333, 78.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.940 - 2.600 |
| R-factor | 0.19347 |
| Rwork | 0.191 |
| R-free | 0.23142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h9v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.718 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.940 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.255 | |
| Number of reflections | 16940 | |
| <I/σ(I)> | 34.3 | 7.9 |
| Completeness [%] | 92.9 | 67.8 |
| Redundancy | 10.4 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.7 M NaCl, 100 mM HEPES |
