5H9V
Crystal structure of Regnase PIN domain, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97899, 0.97928, 0.96405 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.370, 113.370, 187.438 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.490 - 2.750 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.22642 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.490 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 33467 |
| <I/σ(I)> | 22.2 |
| Completeness [%] | 90.5 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1 M (NH4)2HPO4, 200 mM NaCl, 100 mM sodium citrate |
