5H9T
Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.676, 127.602, 265.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.890 |
| R-factor | 0.19699 |
| Rwork | 0.194 |
| R-free | 0.25214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5daj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.690 | |
| Number of reflections | 47656 | |
| <I/σ(I)> | 32.46 | 3.5 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 9.3 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | tacsimate,sodium citrate tribasic dihydrate, polyethylene glycol 3350 |
