5H9G

apo-ACP from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	49.596, 49.596, 72.949
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.600
Rwork	0.240
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t8k
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge		0.613
Number of reflections	6091
<I/σ(I)>	8.5	1.4
Completeness [%]	100.0	98.3
Redundancy	2.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	277	0.1 M sodium acetate trihydrate, 3.0 M Sodium Chloride