5H9C

Crystal structure of the ASLV fusion protein core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-22
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	H 3
Unit cell lengths	42.800, 42.800, 119.428
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.809 - 1.783
R-factor	0.1595
Rwork	0.158
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jpr
RMSD bond length	0.009
RMSD bond angle	0.987
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.810	1.820
High resolution limit [Å]	1.780	1.780
Rmerge	0.060	0.708
Number of reflections	7712
<I/σ(I)>	17.38	1.7
Completeness [%]	98.9	80.2
Redundancy	5.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% (w/v) 2-methyl-2,4-pentanediol, 5% (w/v) PEG 8000, and 0.1 M sodium cacodylate