5H91

LbDERA mutant-T29L/F163Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	97.381, 97.381, 119.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 1.770
R-factor	0.1599
Rwork	0.159
R-free	0.17550
RMSD bond length	0.007
RMSD bond angle	1.081
Data reduction software	HKL-2000 (1.8.2_1309)
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.550	1.800
High resolution limit [Å]	1.770	1.770
Rmerge		0.160
Number of reflections	56862
<I/σ(I)>	23.5
Completeness [%]	99.8	100
Redundancy	14	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	ammonium acetate, sodium acetate