5H91
LbDERA mutant-T29L/F163Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.381, 97.381, 119.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.550 - 1.770 |
| R-factor | 0.1599 |
| Rwork | 0.159 |
| R-free | 0.17550 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-2000 (1.8.2_1309) |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.550 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.160 | |
| Number of reflections | 56862 | |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | ammonium acetate, sodium acetate |
