5H8T

Crystal structure of human cellular retinol binding protein 1 in complex with all-trans-retinol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-07-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	37.550, 44.990, 84.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 1.210
R-factor	0.1196
Rwork	0.118
R-free	0.14700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crb
RMSD bond length	0.009
RMSD bond angle	1.194
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10pre_2138)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.350	1.230
High resolution limit [Å]	1.210	1.210
Rmerge		0.500
Number of reflections	44760
<I/σ(I)>	10.6	1.9
Completeness [%]	99.4	98.5
Redundancy	5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	297	0.1 M Bis-Tris, pH 5.5, 25% PEG 6000