5H8D

Crystal structure of an ASC binding nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 41 21 2
Unit cell lengths	97.421, 97.421, 31.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.890 - 1.890
R-factor	0.1721
Rwork	0.169
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	68.890
High resolution limit [Å]	1.890
Number of reflections	12777
<I/σ(I)>	8.77
Completeness [%]	99.2
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	291	0.1 M citric acid at pH 3.5 and 35% PEG3350