5H89
Crystal structure of mRojoA mutant - T16V - P63Y - W143G - L163V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.031, 108.149, 74.000 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 71.390 - 1.760 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.390 |
| High resolution limit [Å] | 1.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 125mM Bis-Tris, 200mM MgCl2 and 25% Peg3350 |
