5H87
Crystal structure of mRojoA mutant - P63H - W143S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.672, 91.509, 84.605 |
| Unit cell angles | 90.00, 108.72, 90.00 |
Refinement procedure
| Resolution | 32.800 - 2.240 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.800 |
| High resolution limit [Å] | 2.240 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 125mM Bis-Tris, and 25% Peg3350 |
