5H86
Human Gcn5 bound to butyryl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-22 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 38.246, 38.246, 186.965 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.810 - 2.080 |
| R-factor | 0.15823 |
| Rwork | 0.156 |
| R-free | 0.19421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z4r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.253 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.810 | 2.154 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.186 | |
| Number of reflections | 9086 | |
| <I/σ(I)> | 16.86 | 5.39 |
| Completeness [%] | 98.1 | 87.88 |
| Redundancy | 6.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 10% (v/v) 2-propanol, 3% glycerol, 100 mM HEPES, pH 7.8, and 11% (w/v) PEG 4,000 |
