5H7V

Structure of full-length extracellular domain of HAI-1 at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9778
Spacegroup name	P 41 21 2
Unit cell lengths	95.620, 95.620, 124.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.090 - 3.820
R-factor	0.2563
Rwork	0.253
R-free	0.32810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2msx
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.090
High resolution limit [Å]	3.820
Number of reflections	5987
<I/σ(I)>	17.47
Completeness [%]	100.0
Redundancy	25.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	1.6M ammonium sulfate, 20% (v/v) glycerol, 0.08M sodium acetate, pH 4.6