5H7R
Structural basis of the flanking zinc-finger motifs crucial for the E3 ligase activity of the LNX1 RING domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-07-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 92.375, 92.375, 84.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.884 - 1.700 |
| R-factor | 0.18162 |
| Rwork | 0.178 |
| R-free | 0.21528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5din |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.295 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.884 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 31064 | |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 17.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MES monohydrate pH 6.0, 0.05 M calcium chloride dihydrate, 45 % polyethylene glycol 200 |
