5H7K

Crystal structure of Elongation factor 2 GDP-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.159, 85.560, 114.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.559 - 1.599
R-factor	0.1723
Rwork	0.171
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.599	1.600
Rmerge	0.077	0.729
Number of reflections	65800
<I/σ(I)>	19.07	2.52
Completeness [%]	99.9	99.7
Redundancy	7.25	7.24
CC(1/2)	0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	10 % PEG 1000, 0.1 M MES-NaOH