5H7J

Crystal structure of Elongation factor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-02-18
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	84.216, 116.131, 189.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.586 - 2.300
R-factor	0.2172
Rwork	0.215
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.389
Number of reflections	82481
<I/σ(I)>	20.23	3.8
Completeness [%]	99.2	98.7
Redundancy	5.13	5.15
CC(1/2)	0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	10 % PEG 6000, 0.1 M MES-NaOH