5H7B

Crystal structure of a repeat protein with five Protein A repeat modules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2016-05-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	80.890, 45.640, 82.140
Unit cell angles	90.00, 105.72, 90.00

Refinement procedure

Resolution	39.534 - 3.100
R-factor	0.2711
Rwork	0.269
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4znc 5coc
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	8687
<I/σ(I)>	7.2
Completeness [%]	96.9	94.2
Redundancy	2.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	296	0.1M sodium acetate pH 4.5, 27.3% PEG MME 2000