5H6Q
Crystal structure of LSD1-CoREST in complex with peptide 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 120.825, 180.488, 233.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.530 |
| R-factor | 0.22456 |
| Rwork | 0.224 |
| R-free | 0.24675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.418 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.857 | |
| Number of reflections | 84828 | |
| <I/σ(I)> | 29.5 | 2.1 |
| Completeness [%] | 99.8 | 97.8 |
| Redundancy | 7.5 | 7 |
| CC(1/2) | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M N-(2-Acetamido) iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate. |
