5H6Q

Crystal structure of LSD1-CoREST in complex with peptide 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-16
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	120.825, 180.488, 233.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.530
R-factor	0.22456
Rwork	0.224
R-free	0.24675
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.010
RMSD bond angle	1.418
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.570
High resolution limit [Å]	2.530	2.530
Rmerge		0.857
Number of reflections	84828
<I/σ(I)>	29.5	2.1
Completeness [%]	99.8	97.8
Redundancy	7.5	7
CC(1/2)		0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M N-(2-Acetamido) iminodiacetic acid, 1.23 M potassium sodium tartrate tetrahydrate.