5H6N
DNA targeting ADP-ribosyltransferase Pierisin-1, autoinhibitory form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.070, 110.500, 133.640 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 30.068 - 1.800 |
| R-factor | 0.22487 |
| Rwork | 0.224 |
| R-free | 0.24141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cb4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 132451 |
| <I/σ(I)> | 19.3 |
| Completeness [%] | 95.0 |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 22% (v/v) t-butanol and 0.1M Tris-HCl pH8 |
