5H68
Crystal structure of an engaged dimer of the Geobacillus stearothermophilus SMC head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.339, 65.835, 77.923 |
| Unit cell angles | 90.00, 108.11, 90.00 |
Refinement procedure
| Resolution | 46.908 - 1.975 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1300002083 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.975 | 1.980 |
| Rmerge | 0.056 | 0.373 |
| Number of reflections | 46908 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.7 | 84.3 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES (pH 7.5), 0.2 M sodium chloride and 25%(w/v) PEG 3,350 |
