5H66
Crystal structure of the Bacillus subtilis SMC head domain complexed with the cognate ScpA C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.151, 46.905, 97.194 |
| Unit cell angles | 90.00, 112.65, 90.00 |
Refinement procedure
| Resolution | 34.597 - 1.822 |
| R-factor | 0.211745635755 |
| Rwork | 0.211 |
| R-free | 0.22753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xew |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.735 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.050 | 0.258 |
| Number of reflections | 65862 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 3.5M sodium formate (pH 7.4) |
