5H65

Crystal structure of human POT1 and TPP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97876
Spacegroup name	P 21 21 21
Unit cell lengths	49.231, 87.824, 87.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.912 - 2.100
R-factor	0.1988
Rwork	0.196
R-free	0.24810
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.138
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.129	0.058	0.619
Rmeas	0.141	0.063	0.683
Rpim	0.055	0.025	0.283
Total number of observations	139163
Number of reflections	22462
<I/σ(I)>	5.2
Completeness [%]	98.1	99.7	93.6
Redundancy	6.2	6	5.2
CC(1/2)		0.996	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	277	2.0 M NaH2PO4/K2HPO4 (2:3), 0.2 M sodium citrate, 0.1 M acetate pH 4.4