5H63
Structure of Transferase mutant-C23S,C199S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.570, 52.280, 143.750 |
| Unit cell angles | 90.00, 90.00, 108.00 |
Refinement procedure
| Resolution | 44.800 - 1.920 |
| R-factor | 0.18621 |
| Rwork | 0.184 |
| R-free | 0.22800 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.932 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 94014 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 94.4 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 7.5 | 290 | 0.1M HEPES-NaOH (pH 7.5), 0.005M MnCl2, 0.1M sodium acetate, 24%(w/v) PEG 4000 |
