5H62
Structure of Transferase mutant-C23S,C199S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.590, 143.630, 52.730 |
| Unit cell angles | 90.00, 108.24, 90.00 |
Refinement procedure
| Resolution | 47.290 - 1.660 |
| R-factor | 0.18221 |
| Rwork | 0.181 |
| R-free | 0.21265 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.997 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.660 |
| Number of reflections | 77784 |
| <I/σ(I)> | 16.3 |
| Completeness [%] | 99.5 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 6.5 | 290 | 0.1M Bis-Tris (pH 6.5), 0.005M MnCl2, 26% (w/v) PEG 3350 |
