5H61
Structure of Transferase mutant-C23S,C199S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.610, 145.580, 55.480 |
Unit cell angles | 90.00, 105.12, 90.00 |
Refinement procedure
Resolution | 72.790 - 1.860 |
R-factor | 0.19973 |
Rwork | 0.198 |
R-free | 0.23335 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.771 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 72.800 |
High resolution limit [Å] | 1.860 |
Number of reflections | 55555 |
<I/σ(I)> | 15.8 |
Completeness [%] | 98.0 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION, RECRYSTALLIZATION | 6.5 | 290 | 0.1M Bis-Tris (pH 6.5), 26% (w/v) PEG 3350 |