5H5W

Crystal structure of the flagellar cap protein FliD D2-D3 domains from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-06
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00004
Spacegroup name	P 6
Unit cell lengths	118.326, 118.326, 59.581
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.21297
Rwork	0.211
R-free	0.25737
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h5v
RMSD bond length	0.014
RMSD bond angle	1.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.076
Number of reflections	26548
<I/σ(I)>	36.3
Completeness [%]	99.8
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.4 M ammonium sulfate, 0.1 M MES pH 6.5