5H5V
Crystal structure of the flagellar cap protein FliD D1-D2-D3 domains from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.062, 181.582, 110.456 |
| Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.22923 |
| Rwork | 0.228 |
| R-free | 0.25781 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.358 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.088 | |
| Number of reflections | 61902 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES pH 7.0-7.4, 14-18% PEG 6000 |
