5H5J
Complex between ferredoxin and ferredoxin-NADP+ reductase from maize root
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.718, 126.576, 59.367 |
| Unit cell angles | 90.00, 114.82, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h59 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.490 |
| Number of reflections | 91157 |
| <I/σ(I)> | 25.7 |
| Completeness [%] | 99.4 |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20 % PEG6000, 100 mM Sodium cacodylate |
