5H5I
Staphylococcus aureus FtsZ-GDP R29A mutant in R state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-16 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.757, 59.279, 81.770 |
Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
Resolution | 42.570 - 1.900 |
R-factor | 0.1906 |
Rwork | 0.189 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h5g |
RMSD bond length | 0.014 |
RMSD bond angle | 1.695 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.064 | 0.044 | 0.503 |
Rmeas | 0.075 | ||
Rpim | 0.039 | ||
Total number of observations | 86369 | ||
Number of reflections | 22985 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 99.2 | 88.2 | 99.5 |
Redundancy | 3.8 | 3.5 | 3.7 |
CC(1/2) | 0.995 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 100mM Tris, 45% w/v PEP629, 300mM KCl |