5H5G

Staphylococcus aureus FtsZ-GDP in T and R states

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-17
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 1 21 1
Unit cell lengths	43.917, 159.023, 44.058
Unit cell angles	90.00, 92.78, 90.00

Refinement procedure

Resolution	44.010 - 2.200
R-factor	0.187
Rwork	0.184
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
RMSD bond length	0.008
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.080	0.033	0.516
Rmeas	0.089
Rpim	0.039
Total number of observations	156805
Number of reflections	30358
<I/σ(I)>	7.8
Completeness [%]	100.0	99.4	100
Redundancy	5.2	5	5.1
CC(1/2)		0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM HEPES, 39% w/v PEP629