5H5F
The crystal structure of the yeast arginine methyltransferase SFM1 complexed with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.712, 62.417, 44.695 |
| Unit cell angles | 90.00, 112.31, 90.00 |
Refinement procedure
| Resolution | 34.471 - 1.700 |
| R-factor | 0.1548 |
| Rwork | 0.154 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c77 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.100 |
| Number of reflections | 21761 | |
| <I/σ(I)> | 24.5 | 9.6 |
| Completeness [%] | 95.5 | 95.5 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M NaCl, 0.1 M Tris-HCl (pH 8.5), 38% PEG 3350 |
