5H5E
The crystal structure of the yeast arginine methyltransferase SFM1 complexed with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.731, 58.035, 43.357 |
| Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
| Resolution | 37.155 - 2.090 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c77 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.089 | 0.496 |
| Number of reflections | 10918 | |
| <I/σ(I)> | 23.7 | 3.4 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M NaCl, 0.1 M Tris-HCl (pH 8.5), 38% PEG 3350 |
