5H5C
Mdm12 from K. lactis (1-239), uniformly Lys dimethyl modified, crystallized in FOS-MEA-10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-12 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 93.391, 93.391, 81.129 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.310 |
R-factor | 0.29826 |
Rwork | 0.296 |
R-free | 0.33512 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.310 |
Number of reflections | 535031 |
<I/σ(I)> | 15.6 |
Completeness [%] | 99.6 |
Redundancy | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.26M NaH2PO4 0.14M K2HPO4 pH5.6 0.55mM FOS-MEA-10 |