5H4V
Structure of glutamyl-tRNA synthetase (Xoo1504) from Xanthomonas oryzae pv. oryzae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-05-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.96 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.353, 107.937, 165.895 |
| Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
| Resolution | 29.440 - 3.000 |
| R-factor | 0.204 |
| Rwork | 0.200 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cfo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.598 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.087 | 0.310 |
| Number of reflections | 59553 | |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 90.6 | 65.7 |
| Redundancy | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 55%(v/v) Tacsimate, 0.1M bistris propane pH 6.5 |
