5H4E
Crystal structure of a beta-1,3-glucanase domain (GH64) from Clostridium beijerinckii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-01-31 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.758, 72.838, 56.456 |
| Unit cell angles | 90.00, 103.85, 90.00 |
Refinement procedure
| Resolution | 23.637 - 1.863 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gd0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.045 | 0.228 |
| Number of reflections | 28957 | |
| <I/σ(I)> | 40.6 | |
| Completeness [%] | 95.3 | 83.6 |
| Redundancy | 7.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
