5H3Z
Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.761, 94.784, 157.943 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 42.720 - 2.000 |
| R-factor | 0.17019 |
| Rwork | 0.168 |
| R-free | 0.20359 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.667 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.121 | 0.861 |
| Number of reflections | 172867 | |
| <I/σ(I)> | 25.8 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Tris-HCl, calcium acetate, PEG 3350 |
