5H3I

Crystal Structure of Oryza sativa Acyl-CoA-Binding Protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979158
Spacegroup name	P 21 21 2
Unit cell lengths	100.570, 145.050, 25.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.324 - 2.302
R-factor	0.2244
Rwork	0.223
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5DLN
RMSD bond length	0.002
RMSD bond angle	0.387
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.600	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.170	1.422
Number of reflections	18120
<I/σ(I)>	9.71	1.64
Completeness [%]	89.5	58.3
Redundancy	6.84	6.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1M sodium acetate, 0.10 M MgCl2, 26%(w/v) PEG 4000