5H3H

Esterase (EaEST) from Exiguobacterium antarcticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 3
Unit cell lengths	76.765, 76.765, 68.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.590 - 1.900
R-factor	0.1842
Rwork	0.181
R-free	0.23535
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.971
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	35239
<I/σ(I)>	43
Completeness [%]	99.3
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M ammonium acetate, 0.1M HEPES:NaOH pH 7.5, 27%(w/v) PEG3350